RNA-Seq技术在瘤胃微生物研究中的应用进展

反刍动物瘤胃内多种多样的微生物组成一个复杂的微生态系统,它们在瘤胃内物质消化过程中发挥重要作用。RNA-Seq技术的出现为反刍动物瘤胃微生物的研究提供了前所未有的机遇。本文主要从新一代高通量测序技术出发,阐述RNA-Seq技术在瘤胃微生物代谢酶特性、瘤胃微生物的多样性、瘤胃微生物新功能基因方面的应用,以期为瘤胃微生物的研究提供参考。     

草坪灌溉与排水课程设计的关键环节分析

为了提高草业科学本科生创造性和独立性,要求学生完成喷灌系统课程设计。该课程设计特点是公式多、难记忆、抽象和难掌握。在课程设计过程中,学生经常找不到切入点,不知道各环节计算公式和各环节之间的联系。通过对多年教学过程的总结和概括,分析设计灌水定额和灌水周期、喷头布置和选型、轮灌组划分等关键环节,进一步阐明各关键环节之间的联系,以期指导学生更好掌握草坪灌溉与排水课程,达到满足草坪草用水、节约用水、形成水动景观效果、解决我国水资源紧张等目的。     

中央森林生态效益补偿实施中的问题与对策探讨

根据中央森林生态效益补偿实施的现状，从政策、管理、机制等方面分析实施中存在的问题．提出解决问题的对策措施。     

捕食性昆虫螨类及蜘蛛的捕食作用

文章综述了昆虫、螨类及蜘蛛捕食作用研究的近况，重点介绍了昆虫及捕食性螨类和蜘蛛的捕食功能反应，血清学法研究捕食作用的概况，分析了ELISA法的优缺点，并总结了捕食作用的研究现状，指出了面临的问题及发展方向。     

地带性木本植物群落功能型的水热分布格局

植物功能型是对环境条件做出相似的反应和对关键生态学过程具有相似影响的植物组合.不同功能型植物对生态系统过程的作用不同,通过植物功能型的分析对揭示生态系统功能至关重要.甘肃省境内5种地带性木本植物群落(亚热带常绿落叶阔叶林、暖温带落叶阔叶林、温带针阔混交林、寒温性针叶林和荒漠旱生灌木群落)依据植物体的高度、叶片的生长对策及果实类型可划分出23种功能型.其分布格局与水热因子的相关分析表明:1)湿热的生境利于乔木、大灌木、藤木、寄生植物和常绿植物及翅果、坚果、核果、NFDA5NFDA6果、颖果类植物的发育,干冷生境利于灌木、小灌木和落叶植物以及蒴果类植物的发育.2)干果类和肉果类的成因可能与植物生长环境中水分供应状况密切相关,即潮湿的生境利于肉果类植物的发育,干旱的生境有利于干果类(如胞果)的发育.3)研究区域地带性木本植物群落物种丰富度和功能型的丰富度格局主要受制于水分梯度影响,而良好的水热组合可孕育木本植物群落最丰富的果实类型.     

木荷播种育苗的关键技术

采用随机区组设计,对影响木荷播种苗产量和质量的4个主要因素--播种方法、施肥种类、覆土厚度和播种量进行了系统研究,观测了在4种技术条件下种子的发芽率、苗木的高、地径、主根长、侧根数、苗木总产量和合格苗产量,然后运用数理统计方法,从不同的角度分析了苗木质量和产量指标的变异情况.通过观测分析,总结出一套能保证木荷丰产优质的育苗技术.     

多酚羧酸合成功能高分子材料研究(Ⅱ)——没食子酰化改性纤维素合成及其功能特性试验

以没食子酰基为功能基团,以改性纤维素为骨架合成功能高分子材料没食子酰化改性纤维素(没食子酰基-1,6-己二亚胺基-羟丙基-纤维素,CG):先将纤维素与环氧氯丙烷反应,然后依次接上己二胺桥链和三乙酰基没食子酰基,最后将没食子酰基脱去乙酰基得到目标产物。功能试验表明,1g产物能结合明胶49.5mg,并可再生使用。1g产物络合金属离子的能力:Fe3 53.4mg,Cu2 38.7mg,Hg2 46.5mg,Mn2 6.8mg,Zn2 27.9mg,Pb2 57.2mg。产物在稀酸、稀碱、稀醇和热水中具有稳定性。     

GIS在林业专题制图中的应用

论述了运用于理信息系统软件完成应用遥感技术进行思茅地区样地清查及进行“二项调查”的制衅工作，重点阐明了整个制作过程、成果分析、该注意的几点事项。成果得到相关部门的认可，该方法可应用于林业专题图的制作。     

The nameless counterpart: a reconstruction of the experiences of private forestry extension officers with their clients

The changes to the forest ownership structure of small private forests in recent times have opened up an intensive field of research throughout Europe. Most of the studies completed to date adhere to the classic survey model and describe the behaviour and attitudes of forest owners. The research presented in this article analyses the changes to small private forests with the aid of data collected from focus groups comprised of forestry extension officers in Germany. Through the reconstruction of the experiences gleaned by the consultants, it was possible to describe the manner in which they characterise their clientele, and the changes they have observed, against the backdrop of changes occurring across rural areas. The consultants’ strategies for describing forest owners are presented. The orientation pattern presented by the extension officers is compared with the urban orientation of forest owners’ model developed by social scientists.     

The role of indigenous trees for the conservation of biocultural diversity in traditional agroforestry land use systems: The Bungoma case study: In-situ conservation of indigenous tree species

In order to precisely assess the role of agroforestry for the conservation of (agro-)biological diversity there is a need to adjust sampling methods used in plant sociology. This study is on the contribution of agroforestry land use to the in-situ conservation of indigenous trees within a typical East-African smallholder farming system in Western Kenya. A modified approach to measuring species richness and abundance is presented. The selection of tree-rich structures was done according to management patterns rather than considering syntaxonomic classifications from the vegetation science point of view. This approach, which is better suited to the strongly human-influenced vegetation of agroecosystems, employs between-management unit diversity measures to help evaluate abundance and constancy of species in particular management units. The predominant off-farm vegetation patterns are riverine forests, rocky hillsides, hedgerows, wooded grassland relicts, woodlands or colline forest relicts and tree groves whereas the noticeable tree-rich on-farm management units are homegardens, homesteads, life fences, coffee- and banana-groves and annual cropping fields. 98% out of all indigenous tree species known from Bungoma, occur in off-farm lands. 53% of the indigenous tree species occur in tree rich on-farm management units while 47% of the indigenous trees are dependent on the maintenance of off-farm lands. Hedgerows embody the most promising secondary vegetation structure that shows ample potential to accommodate a wide range of indigenous perennial species outside the natural vegetation formations. The pattern of distribution of various vegetation structures, and the mixture with diverse tree-based on-farm plots are interesting features in regard to floristic and eco-diversity on a landscape level. In this respect, three areas that show different population densities and distinct agro-historical conditions were compared. In regions of particular high population density the degree of association of various functional land use forms and woodland structures – each characterised by its own floristic make-up – is comparatively high. In contrast, in regions with less population but that were settled at a later date, one encounters fewer stands of woody plants with lower numbers of species. This revised version was published online in June 2006 with corrections to the Cover Date.